基于微量RNA高通量测序技术的牦牛MII期卵母细胞转录组研究

doi:10.11843/j.issn.0366-6964.2014.05.007

Abstract

Abstract:

The aim of study was established an improved micro-transcriptome sequencing method by using yak oocytes as the trace samples，and provided the basis for the further transcriptome study of yak oocyte.The micro-transcriptome sequencing library were constructed based on the SMART PCR-amplified cDNAs technology by using yak oocytes as the model cell with a starting amount of total RNA as low as 10 ng RNA，and then an improved high-throughput (HT) sequencing technology were applied for transcriptome analysis.After Illumina-Solexa deep sequencing，a total of 47 619 254 clean reads with 4 Gb data were obtained in the micro-oocyte library.Quality control (QC) tests and basic analysis results showed that the sequencing quality of the library was good.The alignment analysis results of the reference genome showed that a total of 15 626 genes from yaks were mapped.Additionally，7 348 novel transcripts that could be positioned back to the yak genome were obtained.The GO analysis results revealed that 13 795 mapped genes took part in three main categories，namely，biological processes，cellular components and molecular functions.Further enrichment analysis results showed that a total of 333 134 and 113 GO categories were enriched above the three main categories.The KEGG analysis results showed that 13 496 mapped genes were involved in 258 pathways，and the enrichment analysis results showed that a total of 101 pathways were enriched.This study established an improved micro-transcriptome sequencing method with yak oocytes as a sample having as low as 10 ng of total RNA.The data not only revealed useful information for future studies on yak genomes，but also provided new insights for the molecular mechanism of the MII stage of the yak oocytes.

CLC Number:

S823.8⁺5.2

LAN Dao-liang，XIONG Xian-rong，LIN Bao-shan，CHEN Ya-bing，HU Min，SU Xiao-shan，LI Jian. Transcriptome Analysis of Yak Metaphase II (MII) Oocytes by a Micro-transcriptome Sequencing Method[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(5): 722-732.

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Transcriptome Analysis of Yak Metaphase II (MII) Oocytes by a Micro-transcriptome Sequencing Method

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